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Hier einige Beispiele aus Friedhelm Achenbachs Zellbiologenphase

Progress in plasmodial differentiation improves regularity of oscillating contractions in Physarum polycephalum.
Helf M; Achenbach F: Cell Biol Int 31, 11-15 (2007).

Based on the knowledge about subcellular morphogenetic processes in the acellular slime mold Physarum polycephalum, we hypothesized that during differentiation of undifferentiated endoplasm to the highly differentiated complex structure of the contractile apparatus of this organism, the regularity of oscillating contractions must improve. We measured the endogenous contraction automaticity starting from the de novo generation within minutes after sampling small portions of undifferentiated endoplasm. The standard deviation of the normalized period duration of these samples was compared to the respective values of radial contractions of differentiated protoplasmic plasmodial strands. The mean normalized standard deviation in endoplasmic drops was 28.3% ± 12.2%. Respective values in protoplasmic strands were 10.0% ± 3.7%. The difference between the experimental groups was highly significant (p<<0.0001). We interpret the verification of our hypothesis as an indication that the very regular oscillating contractions in fully differentiated stages of Physarum require the complex structure of the sophisticated contractile apparatus, represented by the circular plasmalemma invagination system of protoplasmic strands, while the regularity is lower in stages, where the differentiation is still in progress. We believe that this is due to deficits in coordination capabilities, which need a directional and spatially oriented protoplasmic streaming as a precondition. Copyright 2006 Elsevier. Volltext f. Abonnenten, Volltext.

Wirkungen hochfrequenter elektromagnetischer Felder auf planare Lipidmembranen und Zellmembranen lebender Zellen.
Meyer R; Linz K; Achenbach F: In: Berufsgenossenschaft der Feinmechanik und Elektrotechnik (Hrsgb.) Tagungsband - Wirkung hochfrequenter elektromagnetischer Felder auf den Organismus. Eigenverlag Berufsgenossenschaft, Köln, 2000, 26-35.
Protein kinase C affects reformation of endothelial junctions in Xenopus XTH-2 cells.
Werner NS; Meyer R; Achenbach F: Cell Biol Int 23, 73-80 (1999)

Endothelial cells can reversibly be forced to suppress the formation of endothelial junctions (EJ) by cultivation in a low calcium medium. The authors localized vinculin and cadherin as marker proteins of EJ and actin as a cytoskeletal component by fluorescence microscopy, and used this cell model to study the reformation of endothelial junctions under conditions of activation and inhibition of protein kinase C (PKC). Inhibition of PKC by H-7 leads to an acceleration of EJ reformation, while constitutive activation by TPA inhibits the reformation process. Copyright 1999 Academic Press.

Wirkung hochfrequenter elektromagnetischer Felder auf elektrische Eigenschaften künstlicher Membranen mit und ohne eingelagerte Carrier und Kanäle
Friedhelm Achenbach, Klaus Linz, Rainer Meyer (1998). Abschlußbericht.

Es wurde ein Meßaufbau konstruiert, gebaut und in Betrieb genommen, der elektrophysiologische Messungen an künstlichen Lipidmembranen, im weiteren "Bilayer" genannt, unter der Einwirkung hochfrequenter Felder erlaubt. Dieser Aufbau besteht aus einem Hohlleiter in den eine Versuchskammer mit dem Bilayer eingefahren werden kann. Im Inneren des Hohlleiters kann der Bilayer der fortschreitenden Welle eines 900 MHz HF-Feldes ausgesetzt werden. Über dem Bilayer wird eine DC-Spannung angelegt. Es kommt zum Fließen eines sehr kleinen Stromes. Beim Einschalten des HF-Feldes wird eine transiente Steigerung des Stromes unter Spannungsklemmbedingungen festgestellt. Diese Steigerung des Stromes wird im weiteren als Überschußstrom bezeichnet. Der Überschußstrom ist proportional zur Sendeleistung, mit der das HF-Feld erzeugt wird. Er ist umgekehrt proportional zur Fläche der Membran und hängt von der Lage des Bilayers im Feld ab. Der Überschußstrom ist maximal, wenn die Fläche des Bilayers senkrecht zum elektrischen Feldvektor steht. Wesentlichen Einfluß auf die Größe des Überschußstromes hat die Geometrie der Versuchskammer. Allein der Austausch der Standardversuchskammer gegen eine runde führte zu einer signifikanten Verringerung des Effektes auf weniger als 29%. Mögliche Ursachen für den Überschußstrom können eine Veränderung der Leitfähigkeit oder der Kapazität des Bilayers durch das HF-Feld sein. In einer speziellen Versuchsserie wurde versucht, dies zu unterscheiden. Es zeigte sich, daß beim Einschalten des HF-Feldes die Leitfähigkeit des Bilayers sprungartig zunimmt und dann langsam wieder abnimmt. Die Kapazität nimmt dann in etwa mit der gleichen Zeitkonstante zu, mit der die Leitfähigkeit abnimmt. Nach Inkorporation der Antibiotika Alamethicin, Gramicidin und Valinomycin kommt es zu einer Erhöhung der Ladungsverschiebung im Vergleich zu undotierten Membranen. Diese ist am größten bei Gramicidin und Valinomycin. Im Inneren der Versuchskammer ist das HF-Feld ungleich verteilt. Im Bereich des Bilayers kommt es zu einer starken Feldüberhöhung mit Feldern im kV/m Bereich. Damit sind die experimentellen Ergebnisse der Arbeitsgruppen von Prof. Boheim aus Bochum und Prof. Hansen aus Wuppertal im Prinzip reproduziert und das Hauptziel des Projektes ist erreicht. Die grundlegenden molekularen Ursachen für das Verhalten des Bilayers bleiben unklar. Eine direkte Übertragung der hier gewonnen Erkenntnisse auf einen intakten Organismus ist aufgrund der speziellen geometrischen Voraussetzungen für das Auftreten der Überschußströme nicht möglich.

The conductivity of lipid bilayer membranes can be modulated by pulsed radiofrequency fields.
Achenbach F, Linz KW, Detlefsen J, Helminger J, Meyer R (1998) . Abstract Book, 20th Annual Meeting of the Bioelectromagnetics Society, St. Pete Beach, 132.
Changes in electrical characteristics of lipid bilayer membranes induced by pulsed radiofrequency fields.
Linz KW, Achenbach F, Detlefsen J, Helminger J, Meyer R (1998), Abstract Book, 4th EBEA-Congress, Zagreb, 77.
Quantification and preservation of lectin binding by isolated cardiomyocytes.
Meyer R; Kirch D; Achenbach F: Meth Cell Sci, 1997, 19, 101-106

During preparation of cells for experimentation a considerable amount of bound substance is lost. Our aim was to develop a protocol which retained lectin binding to an extent similar to living cells. This procedure would use fixation procedures suited for fluorescent lectin conjugates and gold-conjugates to be visualized by light- and electron microscopy, respectively. We tested glutaraldehyde and paraformaldehyde in different concentrations before and after lectin binding, different buffers and divalent cations, as additives, to determine the effects on preservation of lectin binding. Lectin binding was visualized and semiquantitatively evaluated by image analysis in the light microscope after silver enhancement of lectin-gold conjugates and by using tetramethyl rhodaminyl isothiocyanate (TRITC)-conjugated lectins. Preservation of lectin binding was best visualized with fluorescent lectin conjugates, whereas during silver enhancement procedures of gold-conjugated lectins, a considerable amount of bound lectins was lost. In general, lectin binding to living cells followed by fixation is superior to fixation before lectin binding. Unfavourable combinations of fixatives and buffers can cause a loss of more than 90% bound lectin. In our experiments with freshly isolated guinea pig cardiomyocytes, lectin binding was best when we used Na-cacodylate buffer with glutaraldehyde fixation (0.1%) after binding of lectins to the living cells.

Localization of protein kinase C in primary cultures of human keratinocytes in relation to cell contact proteins.
Blum S; Ness W; Petrow W; Achenbach F: Cell Signal 6, 157-165 (1994)

To investigate the role of protein kinase C (PKC), one of the key factors in cellular signalling, in the integrity of cell contacts, we studied the effects of PKC activation and inhibition on cell-cell and cell-substratum contacts. We localized PKC, actin and two major elements of zonula adherens (vinculin, E-cadherin) and focal contacts (vinculin) in primary cultures of normal human keratinocytes (nHEK) by fluorescent analogue cytochemistry. The activity of PKC was influenced by administration of TPA (12-O-tetradecanoylphorbol-13-acetate, a specific PKC activator) and H-7 [1-(5-isoquinolinesulphonyl)-2-methylpiperazine, a PKC inhibitor] for various periods of time. Our results show varying effects of TPA and H-7 on zonula adherens and focal contacts, suggesting differences in modulation of both types of adherens junctions by mechanisms partially involving PKC. In addition, TPA treatment of nHEK leads to changes in actin cytoskeletal organization.

Reactivation of cytoplasmic actomyosin in Physarum plasmodia extracted with glycerol and dimethylsulphoxide.
Bell R; Achenbach F: J Cell Sci, 1987 Mar, 87 ( Pt 2):, 231-9

Thin-spread plasmodia of Physarum were subjected to extraction procedures using 50% glycerol or DMSO (dimethylsulphoxide) followed by labelling of actin with fluorescent phallotoxins. During the reactivation of the actomyosin system by 2 mM-MgATP fluorescent actin fibres contract isotonically, which results in numerous fluorescent 'contraction beads'. After short-term extraction 1 mM Ca2+ has an inhibitory effect on the reactivation. This calcium sensitivity is abolished after long-term extraction with glycerol. Calcium at 10 mM irreversibly inhibits reactivation, irrespective of the duration of extraction. The inhibitory effect of 10 mM calcium is prevented by phallotoxin labelling prior to incubation in Ca2+. The DMSO model shows an improvement in structural preservation when compared with the glycerol models. However, reactivation is inhibited by prolonged treatment with DMSO.

Reactivation of cell-free models of endoplasmic drops from Physarum polycephalum after glycerol extraction at low ionic strength.
Achenbach F; Wohlfarth Bottermann KE: Eur J Cell Biol, 1986 Apr, 40:2, 135-8

The effect of calcium ions on the reactivation of cytoplasmic actomyosin contraction in cell-free models of endoplasmic drops from Physarum polycephalum after glycerol extraction at low ionic strength depends on the duration of the extraction procedure: Ca++ prevents contraction in 20-h extracted specimens, whereas after several days of extraction this Ca++-sensitivity is lost. These results indicate an inhibitory effect of Ca++ on cytoplasmic actomyosin contraction.

Calcium sensitivity of cytoplasmic actomyosin from Physarum polycephalum following different purification procedures.
Hartsiotis A; Achenbach F; Haugwitz M: Cell Biol Int Rep, 1987 Jul, 11:7, 509-14 

Cytoplasmic actomyosins purified from the acellular slime mold physarum polycephalum by application of two different procedures (Hatano and Tazawa, 1968; Kohama and Kendrick-Jones, 1986) were compared by SDS-PAGE and contraction experiments. In contrast to the 'Hatano actomyosin', 'Kohama actomyosin' contracts in a calcium sensitive manner, i.e., contraction occurs from zero calcium up to pCa4, and is inhibited at greater than or equal to pCa 3. Distinct differences in SDS gels are discussed.

Effect of magnesium on the contractile behavior of actomyosin aggregates isolated from Physarum plasmodia.
Hartsiotis A; Gilges S; Hecks B; Achenbach F: Cell Biol Int Rep, 1988 Apr, 12:4, 245-51

The dependence on calcium concentration of the contractile behavior of actomyosin isolated from Physarum plasmodia according to Kohama & Kendrick-Jones (1986) was investigated under different magnesium conditions. The inhibitory calcium sensitivity is reduced at magnesium concentrations above or below 1 mM, i.e., contraction of actomyosin aggregates is most effectively inhibited in the presence of 1 mM calcium in combination with physiological magnesium concentrations. In the absence of calcium reactivation optimum is obtained at 8.5 mM Mg2+.

Lateral apertures as passage-ways between ectoplasm and endoplasm in plasmodial strands of Physarum.
Wohlfarth Bottermann KE; Achenbach F: Cell Biol Int Rep, 1982 Jan, 6:1, 57-61
An inexpensive "silicone photo device" for transmicroscopic registration of rhythmical movement phenomena
Achenbach F; Achenbach U; Samans KE; Wohlfarth Bottermann KE: Microsc Acta, 1981 Jan, 84:1, 43-50 

A highly sensitive electronic unit (called "silicon photo probe") is described, which enables registration of cellular motion phenomena simultaneous with their light microscopic observation. Changes in light intensity caused by movements of the living object are registered by means of a silicon photo diode (silicon blue cell), which can be mounted within the binocular tube of any type of light microscope replacing one of the oculars. Its application during investigations of oscillating contraction activity in Physarum is reported. Advantages and short-comings are discussed with respect to established photometric, tensiometric and infrared registration techniques.

Ionic currents traverse the slime mould physarum.
Achenbach F; Weisenseel MH: Cell Biol Int Rep, 1981 Apr, 5:4, 375-9 

Self generated electric currents were studied in protoplasmic drops and small plasmodia of Physarum polycephalum with the aid of an extracellularly measuring vibrating electrode. Ionic currents up to 15 µA/cm2 density were found to traverse the objects. In protoplasmic drops current always enters the numerous protrusions and leaves areas with a smooth surface. In monopodial plasmodia current enters the strand and leaves both the advancing front and the retracting end. This result points toward large changes in membrane arrangement or properties occurring during development of plasmodia from protoplasmic drops.

Calcium binding sites in plasmodia of Physarum polycephalum as revealed by the pyroantimonate technique.
Achenbach F; Achenbach U; Kessler D: J Histochem Cytochem, 1984 Nov, 32:11, 1177-84 

Plasmodia of the acellular slime mold, Physarum polycephalum, were treated with an osmium tetroxide fixative containing potassium pyroantimonate to precipitate calcium and thereby localize calcium binding sites and sites of increased calcium concentration. Dense calcium pyroantimonate precipitates were detected within the nucleoli. The distribution of these precipitates during interphase and mitosis coincides with the distribution of the unique minichromosomes in Physarum, i.e., the numerous short pieces of extrachromosomal nucleolar chromatin containing segments of amplified DNA coding for ribosomal RNA. Calcium pyroantimonate precipitates were present as frequent dense granules in the mitochondrial matrix and as fine precipitates in the mitochondrial nucleoid. Large calcium-containing precipitates were seen within cytoplasmic vacuoles, confirming reports by others. In addition, we have identified calcium binding sites along the cytoplasmic surface of the plasma membrane. The distribution of calcium within the plasmodium is discussed in relation to the assembly of the mitotic spindle and the regulation of cell motility.

Hier die Gesamtliste der Fachpublikationen

Full papers (alphabetisch)
    .
  • Achenbach, F. (1987): Reactivation of cytoplasmic actomyosin in cell-free models. In: Nature and Function of Cytoskeletal Proteins in Motility and Transport, 1987 (K.E. Wohlfarth-Bottermann, ed.), Progr. Zool. 34, 109-114, Gustav Fischer Verlag, Stuttgart, New York.
  • Achenbach, F., Achenbach, U. (1979): Oscillating contractions in protoplasmic strands of Physarum: Effects of externally applied ouabain, sodium-, potassium- and calcium-ions. Cell Biol. Int. Rpts. 3, 141-149.
  • Achenbach, F., Achenbach, U., Hartsiotis, A. (1991): Physarum polycephalum - model organism in cell motility research. Physarum Newsletter 22, 44-51.
  • Achenbach, F., Achenbach, U., Kessler, D. (1984): Calcium binding sites in plasmodia of Physarum polycephalum as revealed by the pyroantimonate technique. J. Histochem. Cytochem. 32, 1177-1184.
  • Achenbach, F., Achenbach, U., Samans, K.E., Wohlfarth-Bottermann, K.E. (1981): An inexpensive 'silicon photo device' for transmicroscopic registration of rhythmical movement phenomena. Microscopica Acta 84, 43-50.
  • Achenbach, F., Achenbach, U., Wohlfarth-Bottermann, K.E. (1979): Plasmalemma invaginations, contraction and locomotion of normal and caffeine-treated protoplasmic drops of Physarum. Europ. J. Cell Biol. 20, 12-23.
  • Achenbach, F., Achenbach, U., Wohlfarth-Bottermann, K.E. (1981): Calcium accumulation in plasmodia of Physarum polycephalum. Cell Calcium 2, 587-599.
  • Achenbach, F., Naib-Majani, W., Wohlfarth-Bottermann, K.E. (1979): Plasmalemma invaginations of Physarum polycephalum dependent on the nutritional content of the plasmodial environment. J. Cell Sci. 36, 355-359.
  • Achenbach, F., Weisenseel, M.H. (1981): Ionic currents traverse the slime mould Physarum. Cell Biol. Int. Rpts. 5, 375-379.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1981): Morphogenesis and disassembly of the circular plasmalemma invagination system in Physarum polycephalum. Differentiation 19, 179-188.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1986a): Reactivation of cell-free models of endoplasmic drops from Physarum polycephalum after glycerol extraction at low ionic strength. Europ. J. Cell Biol. 40, 135-138.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1986b): Successive contraction-relaxation cycles experimentally induced in cell-free models of Physarum polycephalum. Europ. J. Cell Biol. 42,111-117.
  • Bell, R., Achenbach, F. (1987): Reactivation of cytoplasmic actomyosin in Physarum plasmodia extracted with glycerol and DMSO. J. Cell Sci. 87, 231-240.
  • Blum, S., Ness, W., Petrow, W., Achenbach, F. (1994): Localisation of protein kinase C in primary cultures of human keratinocytes in relation to cell contact proteins. Cell. Signalling 6, 157-165.
  • Hartsiotis, A., Achenbach, F., Haugwitz, M. (1987): Calcium sensitivity of cytoplasmic actomyosin from Physarum polycephalum following different purification procedures. Cell Biol. Int. Rpts. 11, 509-514.
  • Hartsiotis, A., Gilges, S., Hecks, B., Achenbach, F. (1988): Effect of magnesium on the contractile behavior of actomyosin aggregates isolated from Physarum plasmodia. Cell Biol. Int. Rpts. 12, 245-251.
  • Helf, M., Achenbach, F. (2007): Progress in plasmodial differentiation improves regularity of oscillating contractions in Physarum polycephalum. Cell Biol. Int. 31, 11-15 (2007).
  • Kessler, D., Achenbach, F. (1985): Change of contractile behavior of Physarum polycephalum during mitosis. Protoplasma 124, 71-79.
  • Kukulies, J., Stockem, W., Achenbach, F. (1985): Distribution and dynamics of fluorochromed actin in living stages of Physarum polycephalum. Europ. J. Cell Biol. 35, 235-245.
  • Naib-Majani, W., Achenbach, F., Weber, K., Wohlfarth-Bottermann, K.E. (1984): Immuncytochemistry of the acellular slime mould Physarum polycephalum. IV. Differentiation and dynamics of the polygonal actomyosin system. Differentiation 26, 11-22.
  • Meyer, R., Kirch, D., Achenbach, F. (1997): Quantification and preservation of lectin binding in isolated cardiomyocytes. Meth. Cell Sci., Meth Cell Sci 19, 101-106
  • Werner, N.S., Achenbach, F. (1999): Protein kinase C affects reformation of endothelial junctions in Xenopus XTH-2 cells. A fluorescent analogue cytochemical study. Cell Biol. Int. 23, 73-80.
  • Wohlfarth-Bottermann, K.E., Achenbach, F. (1982): Lateral apertures as passage-ways between ectoplasm and endoplasm in plasmodial strands of Physarum. Cell Biol. Int. Rpts. 6, 57-61.
  • Wolke, A., Briegleb, W., Achenbach, F. (1990): Gravitaxis in Physarum polycephalum. Proc. Fourth Europ. Sympos. Life Sci. Res. Space, ESA SP-307, 547-549.
  • Wolke, A., Niemeyer, F., Achenbach, F. (1987): Geotactic behavior of the acellular myxomycete Physarum polycephalum. Cell Biol. Int. Rpts. 11, 525-528.

Filmveröffentlichungen
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1985a): Zellbiologische Studien an Physarum polycephalum - Ein Modell zur Untersuchung cytoplasmatischer Actomyosine. IWF Film C 1576 des Institut für den Wissenschaftlichen Film, Göttingen.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1985b): Zellbiologische Studien an Physarum polycephalum. Morphogenese und Differenzierung im Protoplasmatropfen. IWF Film C 1543 des Institut für den Wissenschaftlichen Film, Göttingen.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1985c): Cellbiological studies on Physarum polycephalum - Model object for the analysis of cytoplasmic actomyosin. IWF Film C 1576 of the Institut für den Wissenschaftlichen Film, Göttingen.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1985d): Cellbiological studies on Physarum polycephalum - Morphogenesis and differentiation in protoplasmic drops. IWF Film C 1543 of the Institut für den Wissenschaftlichen Film, Göttingen.

Vorträge / Tagungsveröffentlichungen
  • Achenbach, F. (1979): Morphogenetic events in protoplasmic drops of Physarum polycephalum. Europ. J. Cell Biol. 20, 118. Achenbach, F. (1985a): Cell-free models of Physarum polycephalum: the role of calcium in regulation of cytoskeletal components. First International Congress on the Molecular Biology of Physarum polycephalum.
  • Achenbach, F. (1985b): Reactivation of cell-models derived from glycerol-extracted endoplasmic drops of Physarum polycephalum. Europ. J. Cell Biol. 36, Suppl. 7, 3.
  • Achenbach, F., Linz, K., Detlefsen, J., Helminger, J., Meyer, R.: The conductivity of lipid bilayer membranes can be modulated by pulsed radiofrequency fields. Annual Meeting of the Biolectromagnetic Society.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1984): Development investigated and documented by time-lapse cinematography. Morphogenesis and differentiation in protoplasmic drops in Physarum polycephalum. Third International Congress on Cell Biology, Tokyo, Japan, eds. S. Seno and Y. Okada, The Japan Society for Cell Biology.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1985a): Cell biological studies in Physarum polycephalum - A model to investigate cytoplasmic actomyosin. First International Workshop on the Molecular Biology of Physarum polycephalum.
  • Achenbach, F., Wohlfarth-Bottermann, K.E. (1985b): Cell biological studies in Physarum polycephalum - Morphogenesis and differentiation in protoplasmic drops. First International Workshop on the Molecular Biology of Physarum polycephalum.
  • Achenbach, F., Wohlfarth-Bottermann, K.E., Hard, T. (1985a): Physarum polycephalum - model object in contraction physiology. Europ. J. Cell Biol. 36, Suppl. 7, 3.
  • Achenbach, F., Wohlfarth-Bottermann, K.E., Hard, T. (1985b): Morphogenesis and differentiation in protoplasmic drops of Physarum polycephalum. Europ. J. Cell Biol. 36, Suppl. 7, 3.
  • Achenbach, F., Achenbach, U., Hartsiotis, A. (1987): Inhibitory calcium regulation of cell-free models and cytoplasmic actomyosin prepared from Physarum plasmodia. Physarum Conference 1987, Berkeley, California, USA.
  • Achenbach, F., Achenbach, U., Hartsiotis, A., Gilges, S. (1989): Regulation of cytoplasmic actomyosin contraction in Physarum. The Molecular Cell Biology of Physarum polycephalum. Joint Seminar under Japan-U.S. Cooperative Programme, Okazaki, Japan. Physarum News Letter, Okazaki Supplement 1989, 9.
  • Achenbach, U., Achenbach, F. (1985): Calcium binding sites in Physarum polycephalum - possible role in understanding the calcium regulation of cytoskeletal elements. First International Workshop on the Molecular Biology of Physarum polycephalum.
  • Achenbach, U., Achenbach, F., Kessler, D. (1985): Calcium and cytoskeletal function in Physarum polycephalum. Europ. J. Cell Biol. 36, Suppl. 7, 4.
  • Achenbach, F., Kaiser, H.W., Balcerkiewicz, A., Issberner, U., Kreysel, H.W. (1992): Phorbolester prevents formation of normal intercellular junctions in cultured human keratinocytes. 22nd Annual Meeting of the European Society for Dermatological Research, London, 1992.
  • Achenbach, F., Kaiser, H.W., Balcerkiewicz, A., Petrow, W., O'Keefe, E.J., Kreysel, H.W. (1992): E-cadherin: Ca++-dependent association with adherens junctions and actin filaments in keratinocytes. 22nd Annual Meeting of the European Society for Dermatological Research, London, 1992.
  • Blum, S., Scholz, B., Flucht, C., Ness, W., Kaiser, H.W., Achenbach, F., Kreysel, H.W. (1992): Localization of protein kinase C in human epidermis and primary cultures of keratinocytes in relation to cell contact proteins. 22. Jahrestagung der Arbeitsgemeinschaft Dermatologische Foschung, Mainz.
  • Blum, S., Ness, W., Achenbach, F. (1993): Effects of protein kinase C activation and inhibition on the distribution of cell contact proteins in primary cultures of human keratinocytes. Europ. J. Cell Biol. 60, Suppl. 37, 34.
  • Blum, S., Breier, G., Reichmann, E., Achenbach, F., Risau, W. (1994): Stimulation of vascular endothelial growth factor expression by activated ras in mouse breast epithelial cells. Eur. J. Cell Biol. 63, Suppl. 40, 21.
  • Gilges, S., Achenbach, U., Achenbach, F. (1991): Phosphorylation of Physarum myosin light-chain 1 (LC-1). Proc. Acad. Sci. Univ. Idaho.
  • Grohé, C., Achenbach, F., Meyer, R., Vetter, H., Neyses, L. (1994): Maturation of N-linked surface molecules may determine muscle differentiation. 67th Session fo the American Heart Association, Dallas, Texas.
  • Grohé, C., Komodromos, N., Meyer, R., Achenbach, F., Neyses, L., Vetter, H. (1994): Influence of glycosylation on myogenic growth and differentiation. XIIth World Congress of Cardiology of the European Society of Cardiology.
  • Hambach, U., Achenbach, F., Linz, K.W., Meyer, R.: Inactivation of N-type Ca2+ current in SH-SY5Y neuroblastoma cells. Hartsiotis, A., Achenbach, F. (1990): Recovery of the "inverse" calcium sensitivity of endoplasmic drops from Physarum polycephalum. Europ. J. Cell Biol. 51, Suppl. 30, P 7.11.
  • Hartsiotis, A., Gilges, S., Achenbach, F. (1988): The influence of calcium and magnesium on the contractile behavior and ATPase activity of Physarum actomyosin. Biol. Chem. Hoppe-Seyler, 369, 1087.
  • Hartsiotis, A., Gilges, S., Achenbach, F. (1989): Myosin as a calcium regulation site within the actomyosin complex isolated from Physarum plasmodia. Europ. J. Cell Biol. 48, Suppl. 26, 67.
  • Hartsiotis, A., Hecks, B., Gilges, S., Achenbach, F. (1988): Effect of calcium and magnesium on the contractile behavior of actomyosin aggregates prepared from Physarum plasmodia. Europ. J. Cell Biol. 46, Suppl. 22, 64.
  • Kaiser, H.W., Hinz, V.C., Ness, W., Achenbach, F., Petrow, W., Kreysel, H.W. (1991): Uvomorulin: A member of the cadherin family is concentrated at sites of cell-cell contacts in keratinocytes. 29. Jahrestagung der Arbeitsgemeinschaft Dermatologische Forschung, Graz, 1991.
  • Kessler, D., Achenbach, F. (1984): Change of contractile behavior in Physarum polycephalum during mitosis. J. Cell Biol. 99, 183a. Komodromos, N., Grohé, C., Meyer, R., Neyses, L., Achenbach, F. (1994): The influence of glycosylation inhibitors on myogenic differentiation of skeletal muscle cells in vitro. Eur. J. Cell Biol., 63, 227.
  • Linz, K., Achenbach,F., Detlefsen, J., Helminger, J., Meyer, R. (1998): Changes in electrical characteristics of lipid bilayer membranes induced by pulsed radiofrequency fields. 4th EBEA Congress Zagreb.
  • Meyer, R., Kirch, D., Achenbach, F. (1994): The influence of fixation procedures on lectin binding to cardiomyocytes. Eur. J. Cell Biol. 63, 228.
  • O'Keefe, E.J, Kaiser, H.W., Hinz, V.C., Achenbach, F., Kreysel, H.W. (1992): Adherens junctions and desmosomes: two types of cell-cell adhesion structure in epidermis.
  • Werner, N.S., Achenbach, F.: Reformation of the endothelial junction in Xenopus cardiac endothelial (XTH-2) cells is influenced by protein kinase C activity. Europ. J. Cell Biol., supplement 43, 201.
  • Werner, N.S., Winkelhaus, S., Hauser, M., Achenbach, F. (1997): Effects of the tyrosine kinase inhibitor 6-DMAP on cytoskeleton, mitotic spindles and focal contacts in Xenopus cardiac endothelial (XTH-2) cells. Europ. J. Cell Biol., supplement 43, 202.
  • Winkelhaus, S., Werner, N., Hoppen, R., Mittag, N., Hauser, M., Achenbach, F. (1996): Effects of calcium, TPA and H-7 on cytoskeleton and cell contacts in Xenopus cardiac endothelial (XTH-2) cells. Europ. J. Cell Biol., supplement 42, 182.
  • Wolke, A., Achenbach, F. (1988): Plasmodial geotaxis in Physarum polycephalum. Europ. J. Cell Biol. 46, Suppl. 22, 232.
  • Wolke, A., Block, I., Briegleb, W., Achenbach, F. (1993): Contraction frequency of Physarum polycephalum analyzed by Fourier-Transformation: A comparison between the classical and a new computerized numerical method. Europ. J. Cell Biol. 60, Suppl. 37, 316
  • Wolke, A., Briegleb, W., Achenbach, F. (1990): Gravitaxis in Physarum polycephalum. Fourth European Symposium on Life Science in Space. ESA SP-307.

Sonstige
  • Achenbach, F., Achenbach, U., Kessler, D. (1985): Quantitative Cytochemie mit dem interaktiven Bildanalyse-System IBAS II am Beispiel von Calcium-Bindungsstellen im Zellkern des Schleimpilzes Physarum polycephalum. Zeiss Information 97, 42-44.
  • Achenbach, F., Achenbach, U., Kessler, D. (1986a): Quantitative Cytochemistry of nuclear calcium binding sites in Physarum polycephalum. Application of the interactive image processor IBAS II. Zeiss Information 97, 42-44.
  • Achenbach, F., Achenbach, U., Kessler, D. (1986b): Bildanalyse von Calcium-Bindungsstellen im Zellkern. Bioengineering 4, 36-38.
  • Achenbach, F., Tommelstad, O. (1981): Wissenschaftliche Fotografie mit der Hasselblad 2000 FC. Hasselblad 69, 24-25.
  • Achenbach, F. (1987): Myxomyceten - Pilz und Amöbe. Lebenslauf echter Schleimpilze. Tonkommentar und Begleitpublikation zum gleichnahmigen Film von G. Schimanski; Inst. f. Film u. Bild in Wissensch. u. Unterr. 3203827.

Dissertationen
  • Hartsiotis, A. (1990): Biochemische und kontraktionsphysiologische Untersuchungen zur Regulation der Actin-Myosin-Interaktion bei Physarum polycephalum. Dissertation an der Math.-Nat. Fakultät der Universität Bonn.
  • Ness, W. (1995): Bedeutung von Komponenten des Membranzytoskeletts und des Aktomyosinsystems für die mechanische Stabilisierung von Keratinocyten und Gefässendothelzellen. Dissertation in Zusammenarbeit mit der Universität Würzburg.
  • Pohl, T. (1990): Strömungsmechanisches Modell einer reaktiven Zweiphasenflüssigkeit. Dissertation in Zusammenarbeit mit dem Institut für Botanik, Abteilung Theoretische Biologie, an der Math.-Nat. Fakultät der Universität Bonn.
  • Trube, A. (1992): Immuncytochemische Darstellung des myotropen Neuropeptids CCAP im Nervensystem von Flußkrebsen. Dissertation in Zusammenarbeit mit dem Institut für Zoophysiologie an der Math.-Nat. Fakultät der Universität Bonn.
  • Wolke, A. (1993): Gravitationsbiologische Untersuchungen an Physarum polycephalum. Laufende Dissertation an der Math.-Nat. Fakultät der Universität Bonn in Kooperation mit dem Institut f. Flugmedizin der Deutschen Forschungsanstalt für Luft- und Raumfahrt (DLR), Porz.

Diplomarbeiten
  • Blum, S. (1994): Regulation des vaskulären Endothelzell-Wachstumsfaktors (VEGF) in Tumorzellen. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn in Kooperatin mit dem MPI f. Physiologische und Klinische Forschung, WG Kerckhoff Institut, Bad Nauheim.
  • Feltgen, J. (1994): Restriktionsenzyme und DNA Doppelstrangbrüche. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn in Kooperation mit der Radiologischen Universitätsklinik, Experimentelle Radiologie u. Strahlenbiologie der Univ. Bonn.
  • Gilges, S. (1990): Biochemische Untersuchungen zu der Regulation von Physarum Myosin. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn.
  • Hartsiotis, A. (1987): Extraktionseffekte bei der Herstellung zellfreier Modelle von Physarum polycephalum. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn.
  • Hinz, V.C. (1991): Nachweis und Lokalisation von Uvomorulin in normalen humanen Keratinozyten und A431-Zellen. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn.
  • Honer, M. (1993): Untersuchungen zur Wirkung von Cytostatika auf Langzeit- und Kurzzeitkulturen von Neuroblastomzellen. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn in Kooperation mit der Medizinischen Fakultät der Universität Köln.
  • Kappe, S. (1991): Untersuchungen zur Motilität, Invasion und der Parasit-Wirtszell-Beziehung von Sporozoiten und Merozoiten der Gattung Eimeria und Sarcocystis (Sporozoa, Coccidia) mit Hilfe videomikroskopischer und laserrastermikroskopischer Techniken. Diplomarbeit in Kooperation mit dem Institut für Zoologie an der Math.-Nat. Fakultät der Universität Bonn.
  • Komodromos, N. (1993): Untersuchungen zur Differenzierung von Fibroblasten zu Myoblasten und Muskelzellen. Diplomarbeit in Kooperation mit dem Institut f. Physiologie II der Med. Fakultät der Universität Bonn.
  • Lichte, R. (1994): Untersuchungen zur Bedeutung von Glycosylierungsreaktionen bei der myogenen Differenzierung. Diplomarbeit in Kooperation mit der Universitäts-Poliklinik der Med. Fakultät der Universität Bonn.
  • Patz, M. (1987): Mikroskopische Untersuchungen zur Darstellung von Actomyosinfibrillen in Physarum polycephalum. Diplomarbeit in Kooperation mit dem Fachbereich "Fotoingenieurwesen" der Fachhochschule Köln.
  • Ramos, A. (1995): Immuncytochemische Untersuchungen zur Bildung von Seitenwurzeln bei Zea mays L. Diplomarbeit in Kooperation mit dem Institut für Botanik an der Math.-Nat. Fakultät der Universität Bonn.
  • Reichelt, S. (1992): Charakterisierung der Mikronemen und apikaler Antigene der Merozoiten von Eimeria nieschulzi (Protozoa, Coccidia). Diplomarbeit in Kooperation mit dem Institut für Zoologie an der Math.-Nat. Fakultät der Universität Bonn.
  • Welzel, M. (1995): Der Einfluß von Glykosilierungsinhibitoren auf die Differenzierung von Skelettmuskelzellen. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn in Kooperation mit der Universitäts Poliklinik.
  • Werner, N.S. (1997): Zelladhäsionsstrukturen und Cytoskelettorganisation interphasischer und mitotischer XTH-2 Zellen nach Beeinflussung des Phosphorylierungszustandes. Lehrstuhl für Zellmorphologie der Ruhr-Universität Bochum.
  • Werth, P. (1994): Untersuchungen zur Regionalität des Zellwechsels in Epithelien des vorderen Augenpoles. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn in Kooperation mit der Universitäts-Augenklinik Bonn.
  • Wolke, A. (1990): Untersuchungen zum Lokomotionsverhalten von Physarum polycephalum unter dem Einfluß der Schwerkraft. Diplomarbeit an der Math.-Nat. Fakultät der Universität Bonn.